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Image Search Results
Journal: Cell
Article Title: Intrinsic Immunity Shapes Viral Resistance of Stem Cells
doi: 10.1016/j.cell.2017.11.018
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Mouse monoclonal anti-ALB ,
Techniques: Control, Recombinant, Clinical Proteomics, Knock-Out, Membrane, SYBR Green Assay, Sample Prep, Selection, shRNA, Software, Gene Expression
Journal: Frontiers in Immunology
Article Title: Antigen–antibody complex density and antibody-induced HLA protein unfolding influence Fc-mediated antibody effector function
doi: 10.3389/fimmu.2024.1438285
Figure Lengend Snippet: Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) Complement-dependent cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.
Article Snippet:
Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Two Tailed Test, MANN-WHITNEY, Control, Concentration Assay