rabbit igm cedarlane cat Search Results


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Cedarlane low tox m rabbit complement cedarlane cat
Low Tox M Rabbit Complement Cedarlane Cat, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane cl8942ap rrid ab 10060357
Cl8942ap Rrid Ab 10060357, supplied by Cedarlane, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lympholyte M Cedarlane Laboratories Cat, supplied by Cedarlane, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane tbrp1 247 mouse anti ep
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Qiagen dna extraction kit qiagen
Dna Extraction Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hycult Biotech anti c3b antibody
Anti C3b Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane clmcnet
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Cedarlane cl2513a rrid ab 10086438
KEY RESOURCES TABLE
Cl2513a Rrid Ab 10086438, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane baby rabbit complement
Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) <t>Complement-dependent</t> cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.
Baby Rabbit Complement, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Cedarlane anti mouse igg hrp
Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) <t>Complement-dependent</t> cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.
Anti Mouse Igg Hrp, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cedarlane density gradient centrifugation
Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) <t>Complement-dependent</t> cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.
Density Gradient Centrifugation, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Cedarlane anti rabbit igg hrp
Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) <t>Complement-dependent</t> cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.
Anti Rabbit Igg Hrp, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell

Article Title: Intrinsic Immunity Shapes Viral Resistance of Stem Cells

doi: 10.1016/j.cell.2017.11.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-ALB , Cedarlane , Cat# CL2513A RRID:AB_10086438.

Techniques: Control, Recombinant, Clinical Proteomics, Knock-Out, Membrane, SYBR Green Assay, Sample Prep, Selection, shRNA, Software, Gene Expression

Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) Complement-dependent cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.

Journal: Frontiers in Immunology

Article Title: Antigen–antibody complex density and antibody-induced HLA protein unfolding influence Fc-mediated antibody effector function

doi: 10.3389/fimmu.2024.1438285

Figure Lengend Snippet: Characterization of immune-potentiating/pathogenic alloantibodies. (A) Bio-panning workflow for antibody discovery using the Humanyx phage library Created with BioRender.com . (B) Binding reactivity of the eight alloantibodies against 21 Class I alleles tested on ELISA. (C) Complement-dependent cytotoxicity (CDC) using CF863-C*07:02 as target cells. (D) Antibody-dependent cellular cytotoxicity (ADCC) using CF863-C*07:02 as target cells. Cells were incubated with complement serum for CDC or with NK cells (effectors cells) for 4 hr before analysis. Two different concentrations, 5 and 25 μg/mL, were tested for all the antibodies (means ± s.d., N= 4 independent experiments). *p = 0.0286 when analyzed with two-tailed Mann–Whitney test, compared to isotype control human monoclonal anti-dengue antibody 14c10-IgG1 for both CDC and ADCC. (E) Human leukocyte antigen (HLA)–antibody complex on CF863-C*07:02 cell line as measured using QIFIKIT. Results represent four independent experiments. *p = 0.0286, when analyzed with two-tailed Mann–Whitney test, compared to Ab 1B2-IgG1 (highest mean in the non-pathogenic group). (F) Strong correlation was observed between cytotoxicity and antigen–antibody complex density (Pearson’s correlation coefficient, r = 0.9334 for CDC and r = 0.9601 for ADCC). (G) Binding kinetics measured on Bio-layer interferometry (BLI) biosensor. Association and dissociation curves of the eight alloantibodies. Six different concentrations of HLA-A*11:01 and/or HLA-C*07:02 monomers, ranging from 1 μM to 15.6 nM, with two fold dilutions were tested. HLA-B*40:01–strep complex was tested at concentrations ranging from 200 nM to 12.5 nM. Red line shows 1:1 curve fitting for each concentration. Results represent two independent experiments.

Article Snippet: Baby rabbit complement (Cedarlane, Cat. #CL3441-S) at a volume of 5 μL (final concentration 10%) was added to the plate and further incubated for 4 hr at 37°C.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation, Two Tailed Test, MANN-WHITNEY, Control, Concentration Assay